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Insights into Nuclear Dynamics using Live-Cell Imaging Approaches

Insights into Nuclear Dynamics using Live-Cell Imaging Approaches

CRISPR is a newly developed, but rapidly advancing, genome editing technique that has advanced the nuclear imaging field by providing a means to fluorescently label a dead Cas9 protein fused with green fluorescent protein (dCas9-GFP), allowing scientists to microscopically track where certain proteins bind to the DNA.