The CRISPR/Cas9 toolbox has been expanded with fluorescent Cas9 and a dCas9 functionalized with a tag able to activate gene expression that can be transfected to cells directly as ribonucleoprotein complexes (RNP).

The CRISPR/Cas9 toolbox has been expanded with fluorescent Cas9 and a dCas9 functionalized with a tag able to activate gene expression that can be transfected to cells directly as ribonucleoprotein complexes (RNP).
A chemically assisted focused-helium-ion-beam-induced etching process is introduced.